Journal: AIDS Research and Human Retroviruses

Article Title: Working Group Consultation: Alloimmunity as a Vaccine Approach Against HIV/AIDS: National Institutes of Health Meeting Report, May 24, 2012

doi: 10.1089/aid.2013.0041

Figure Lengend Snippet: Principal Components of a Pilot Framework Nonhuman Primate Protocol Drafted in the “Working Group Consultation on Alloimmunity as a Vaccine Approach,” National Institutes of Health Meeting, May 24, 2012

Article Snippet: Key Questions Addressed in the “Working Group Consultation on Alloimmunity as a Vaccine Approach,” National Institutes of Health Meeting, May 24, 2012 table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 NHP protocol components Recommendations NHP subspecies Mauritian cynomolgus macaques (MCM) with well-defined MHC class I and class II alleles Challenge virus Specificity Molecular clone of pathogenic SIV mac239 Stock In vitro (PBMC) and in vivo (infection followed by ex vivo spleen culture) to recover and pool virus from haplotype-matched MCM In vivo titration and biochemical characterization of glycoforms, cytokines, and alloantigens Route Mucosal (rectal) Dose Repeat low-dose exposure for a minimally infectious dose such that the control group is infected by three to four weekly exposures Immunogen Skin graft transplantation Pilot study design Test if MHC disparate skin graft in male MCM leads to alloimmunization against mucosal SIV mac239 Immune responses Functional profiles of humoral, cellular, and innate immune cells; viremia Assays and reagents resources MHC class I/II tetramers coupled flow cytometry and ELISpot for specificity, phenotype, and quantitation of allo-T and B cell responses Feasible next steps if protection from acquisition is observed Collected serum use for passive transfer NHP studies to elucidate antibody-mediated protection against challenge Identification of human cohorts/HLA typing databases to complement the protective effect of alloimmunization with epidemiologic studies Open in a separate window NHP, nonhuman primate; PBMC, peripheral blood mononuclear cells.

Techniques: Virus, In Vitro, In Vivo, Infection, Ex Vivo, Titration, Control, Transplantation Assay, Functional Assay, Flow Cytometry, Enzyme-linked Immunospot, Quantitation Assay